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American Society of Microbiology ABMM Exam Syllabus Topics:

American Society of Microbiology ABMM Sample Questions:

1. Lyme disease, characterized initially by erythema migrans, is caused by Borrelia burgdorferi. What is the primary arthropod vector responsible for transmitting this spirochete to humans in the Northeastern United States?

A) Ixodes scapularis (Blacklegged tick)
B) Dermacentor variabilis (American dog tick)
C) Pulex irritans (Human flea)
D) Amblyomma americanum (Lone Star tick)

2. A patient with a history of neutropenia following chemotherapy develops a rapidly progressive pneumonia.
Bronchoalveolar lavage fluid reveals septate hyphae with acute angle (45-degree) branching. The MOST likely diagnosis is invasive pulmonary aspergillosis caused by:

A) Aspergillus fumigatus
B) Rhizopus species
C) Mucor species
D) Fusarium species

3. Microscopic examination of a potassium hydroxide (KOH) preparation of skin scrapings from a patient with tinea versicolor typically reveals:

A) Septate hyphae and arthroconidia
B) Broad, aseptate hyphae with sporangiophores
C) Short, unbranched hyphae and globose yeast cells in clusters ("spaghetti and meatballs")
D) Cigar-shaped yeast cells

4. A patient with HIV infection and a CD4+ T-cell count below 50 cells/µL develops a progressive neurological illness characterized by confusion, motor deficits, and seizures. Brain MRI reveals multiple white matter lesions without mass effect. PCR of the CSF is positive for JC virus. Thepathogenesis of this condition, progressive multifocal leukoencephalopathy (PML), involves:

A) Opportunistic infection by JC virus due to impaired cell-mediated immunity.
B) Lytic infection of oligodendrocytes by JC virus, leading to demyelination.
C) Direct cytopathic effect of JC virus on neurons.
D) Inflammatory demyelination triggered by JC virus infection of astrocytes.

5. A clinical microbiology laboratory is implementing a new real-time PCR assay for the direct detection of Chlamydia trachomatis in urine samples. To ensure the accuracy of negative results, it is essential to include:

A) A positive control containing Neisseria gonorrhoeae DNA.
B) A high concentration of the Chlamydia trachomatis target sequence.
C) A melting curve analysis to confirm the specificity of the amplicon.
D) An internal amplification control to detect PCR inhibition.

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